RNA for microarrays was collected using RNeasy Mini kits (Qiagen) and amplified and biotin-labeled using the MessageAmp-II Bacteria RNA amplification kit (Ambion) per the manufacturer's instructions; 10 15 ng of template RNA was used per reaction.
High throughput gene expression data from spotted cDNA microarrays are collected by scanning the signal intensities of the corresponding spots by dedicated fluorescence scanners.
The data from the microarray was collected and analyzed in accordance to the MIAME guidelines.
Cultured skin substitutes were grafted on full-thickness wounds in athymic mice, and biopsy samples for microarray analyses were collected at multiple in vitro and in vivo time points.
Culture samples for microarray experiments were collected at exponential, transition, and stationary phases.
Gene expression microarray data were collected for both mouse and human.
Microarray data were collected and analyzed using Agilent G2567AA Feature Extraction software, following Agilent's direct labeling protocol.
Samples used for microarray analysis were collected from cultures in THY and AF at mid logarithmic (ML), late logarithmic (LL) and stationary (S) growth phases.
Blood samples for microarray analysis were collected from each subject in PAXgene™ tubes (Qiagen/BD GmbH, UK), incubated at room temperature for 4 hours for RNA stabilization, and then stored at −80°C.
