Sentence examples for many sequencing errors from inspiring English sources

We argue that a better option is the antibody repertoire database (formed by centers of clusters constructed by I gR epertoireC onstructor) as it eliminates many sequencing errors.

We did not use SeqClean to remove adapter sequences since it works only with 100-92% identity segments and in our case adapters contained many sequencing errors caused by the homopolymer runs.

Of these, 57 were manually curated (Additional file 4 and Additional file 5) as the remainder were found to be either allelic variants of the same P450 gene or contained too many sequencing errors.

Manual inspection of SNP candidates in more than 10,000 contigs indicated that many sequencing errors occurred in the beginning of read sequences which, as a consequence, lead to false positives.

Although the probability of each single base being sequenced incorrectly is quite low, denoted by Phred values up to 41, the large number of single base reads within each data set will induce many sequencing errors [ 16].

To avoid including contigs comprising reads that did not match their correct location because they included too many sequencing errors, contigs from the de novo assembly were aligned by BLAST [ 35] to the reference assembly.

In order to process all ESTs in the same manner and to avoid too many sequence errors included as putative SNPs any difference within the 50 first and 50 last base pairs in the EST sequences were not considered as putative SNPs.

That so many sequences contained sequencing errors in the primers (n = 377) was surprising, and warranted further investigation.

We next examined how many of the sequencing errors altered gene predictions by identifying polymorphisms mapping to exons.

On the other hand, only 21.3% of the pSCHs were validated, indicating that many may represent sequencing errors or mutations introduced during the cDNA synthesis procedure.

Although step 4b) is designed to isolate reads that likely contain errors so they can be ignored (see below), many sequencing technologies induce frequent errors that are easy to identify.