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Therefore, application of TPS may be limited to clones with long inserts or for which many sequencing primers need to be designed.
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The mode of the length distribution of individual sequences is 20 bp, and sequences more frequently occur in multiples of 2, suggesting that many sequences are PCR primers (Supplementary File 4).
Briefly, PCR primers and sequencing primers were designed using PSQ Assay Design software (Qiagen, Inc) to capture the array CpG and as many neighboring CpGs as possible.
Exon-specific primers were designed with sequencing primers, allowing direct sequencing of each VWF exon.
Sequencing primers and PCR primer sequences were the same.
Sequences for bisulphite sequencing primers.
PCR and sequencing primers.
Sequencing primers for pDECKO.
Additional file 5: Sequencing primers.
The first 16S primer set revealed many sequences related to Pelagophyceae and Dictyochophyceae, the second 16S primer set was heavily biased toward Prymnesiophyceae, while 18S sequences were dominated by Prasinophyceae, Chrysophyceae and Haptophyta.
That so many sequences contained sequencing errors in the primers (n = 377) was surprising, and warranted further investigation.
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