Many of the sequencing approaches utilize bisulfite conversion to differentiate methylated from unmethylated cystosines with base resolution [ 6, 7].
Direct cloning, traditional sequencing, and deep sequencing approaches have discovered many non-conserved miRNAs in rice, and their predicted target genes encode a broad range of proteins, including some transcription factors (Supplementary Table 1).
Many genetic typing methods use DNA sequencing approaches, but others use specific gene probes, single-strand conformation polymorphism (SSCP), or restriction fragment length polymorphisms (RFLP).
There are many other instances in which PCR and DNA sequencing approaches provide information about cancer treatment and prognosis.
Large scale shotgun sequencing approaches allow the discovery of many novel genes found in the environments independent of cultivation efforts [5] [8].
New large-scale sequencing approaches have revealed the extensive intra- and intertumour heterogeneity in many cancers [ 40- 42].
Box 1: Sequencing approaches for ecological genomics Whole-genome resequencing is cost-prohibitive and unnecessary in many cases.
Many of the approaches we have outlined will become even more powerful as WGS supersedes WES and other targeted sequencing approaches.
The recent genome-wide sequencing approach for many organisms has resulted in the discovery of many new proteins with increasingly obscure names.
This comprehensive screen for E. coli sRNAs using a deep sequencing approach has shown that many as-yet-undiscovered sRNAs are potentially encoded in the E. coli genome.
