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Many sequencing techniques allow for the generation of reads from the two ends of a DNA fragment simultaneously.
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Whereas classically the three eukaryotic RNA polymerases (Pol I, II and III) had clearly separate responsibilities in the gene expression repertoire, deep sequencing techniques have uncovered many new transcription units for both Pol II and III that often give rise to short lived or regulatory non-coding RNA [6 8].
The advances in sequencing techniques and the accumulation of many protein sequences without the purified protein challenge the development of computer algorithms to identify dominant T-cell epitopes based on sequence data alone.
Genome surveys using conventional sequencing techniques are powerful, particularly for detecting sequences present in many copies per genome.
Modern sequencing techniques allow the reconstruction of the network of biochemical reactions in many organisms, from bacteria to human [ 38, 39].
They are well established in many areas, e.g. MIAME [ 29] for microarrays, while other areas as novel sequencing techniques require further elaboration.
The advent of full genome sequencing techniques led to the discovery that the genome encodes at least as many lncRNAs as the known protein-coding genes.
Advances in the quality and efficiency of sequencing methods, such as the next generation sequencing techniques, will assist GWAS and generate vast quantities of rich data on many thousands of individuals.
sequencing techniques.
Most sequencing techniques require days of work.
DNA is sequenced without cloning, using next-generation sequencing techniques.
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