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Nonetheless, until recently, traditional laboratory methods for the development of EST resources have required costly and time consuming approaches involving cloning, cDNA library construction, and many labor intensive Sanger sequencing runs [ 2].
He devised many sequences of hearty runs and surges across the stage for large and small groups, and the students invested them all with energy and earnestness.
Small studies might sequence the highly expressed genes from many different tissues in the same sequencing runs without the need for bar-coding.
We did not use SeqClean to remove adapter sequences since it works only with 100-92% identity segments and in our case adapters contained many sequencing errors caused by the homopolymer runs.
We would like to acknowledge the Broad Institute's Genome Sequencing Platform for preparing and sequencing many of the 454-FLX and Illumina libraries, and Stuart Levine and his group at the MIT BioMicro Center for performing additional Illumina sequencing runs.
Sequencing runs and mapping summary.
HP and CD performed sequencing runs.
Statistics for high throughput sequencing runs.
We performed 26 paired-end sequencing runs.
Such barcodes allow researchers to analyze digital tags from transcriptomes of many samples in a single sequencing run by simply pooling the libraries.
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