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Moreover, linker fragments can have multiple sequencing errors.
However, if a large number of sequencing reads are available for a given locus, multiple sequencing errors might be likely.
Additionally, there is a small probability of multiple sequencing errors leading to genotype calls that are erroneous but still consistent with IBS*.
Those with low coverage are most likely reads with multiple sequencing errors aligned in the wrong position.
Similar(56)
We next employed Gblocks [ 101, 102] to minimise the impact of multiple sequence alignment errors and divergent regions.
Reads mapping to multiple locations, containing sequencing errors or duplicated reads were discarded from further analysis.
Upon manual inspection of the assembly, we identified some mRNA species that were assembled as multiple contigs because of sequencing errors.
We utilize multiple signals to distinguish sequencing errors from real variants: In the absence of a variant, the frequency of the reads with a nucleotide different from the reference base at a particular position should be similar across multiple pools.
These algorithms are sensitive to multiple sources of error including sequencing errors, incorrect alignment ("mismapping"), and random sampling [ 1– 8].
Candidate SNV sets are typically filtered to remove multiple indicators of sequencing error.
Sequence tags containing random sequencing errors should not occur multiple times in multiple samples and should not map genetically, so they should be filtered out in subsequent steps.
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