Many additional SNPs/indels occur in song sparrows, we describe only those with a high probability of being real, not sequencing artefacts.
This strict and conservative approach was chosen to preclude inclusion of sequences from potential contamination or sequencing artefacts.
Counts for sequencing artefacts were also very low and consistent.
All others were assumed to be sequencing artefacts.
Separating true variation from sequencing artefacts still represents a considerable challenge.
False mutation proportion refers to the sequencing artefacts that occur by chance at each nucleotide position.
The degree of fragmentation and sequencing artefacts differed between blocks sourced from different anatomical pathology laboratories.
This way the possibility of cloning and sequencing artefacts was considered.
In the context of massively parallel sequencing (MPS), distinguishing true low frequency variants from sequencing artefacts remains challenging.
For example, the in-silico SNP-mining process with stringent quality criteria can distinguish sequence variations from sequencing artefacts.
