Sentence examples for primer mixture from inspiring English sources

First-strand cDNA synthesis was carried out by random priming of the total RNA using a random primer mixture (Invitrogen) and reverse transcriptase with superscript 3 (Invitrogen).

Reverse transcription of mRNA was performed with the mouse MGB IDO1 primer mixture (Gene Expression Assays: Mm00492586_m1) and mouse GAPDH primers (Applied Biosystems, Foster City, CA).

Each mutation-specific primer mixture was initially evaluated against both cloned lab-generated and patient-derived mutant virus sequences that were serially diluted 10-fold in backgrounds of wildtype sequence plasmids.

PCR amplifications were conducted in a total volume of 15 µl, comprising of 1 µl of the cDNA, 2 µl of primer mixture, and 12 µl of Plus Master Mix (Qiagen, HotStarTaq Plus).

For each array, total RNA (12.5 µg) obtained for each sample was reverse transcribed and labeled with amino-allyl dUTP using reverse transcriptase III and Oligo-dT/random primer mixture (Invitrogen, Carlsbad, CA).

To construct tau isoform with 383 amino acids, two PCR amplifications were conducted with the primer mixture of TauN and LE10-352, pGEX-2T-Tau352 pGEX-2T-Tau352 pGEX-2T-Tau352l as the mixtemplateE10-up asd TauC, using pET-17b-Tau441 as the template.

To construct tau isoform with 412 amino acids, two PCR amplifications were conducted with the primer mixture of TauN and E2-down, using pET-17b-Tau441 as the template, as well as the mixture of LE2-352 and TauC, using pET-17b-Tau383 as the template.

Briefly, to construct human tau isoforms with 381 and 410 amino acids, two PCR amplifications were conducted with the primer mixture of TauN and E2-down, or E3-down, using pET-17b-Tau441 as the template, as well as the mixture of LE2-352 or LE3-352 and TauC, using pGEX-2T-Tau352 pGEX-2T-Tau352 pGEX-2T-Tau352

The PCR mixture (50 µl final volume) contained the following: 25.5 µl sterile distilled water, 5 µl 10×PCR buffer (500 mmol/l KCl and 100 mmol/l Tris-HCl: pH 8.3), 4 µl of a deoxynucleotide mixture (dGTP, dTTP, dATP, and dCTP; 2 mmol/l each), 5 µl MgCl2 (2.5 mmol/l), 5 µl of a primer mixture (10 µmol/l each), 5 µl template DNA, and 0.5 µl AmpliTaq DNA polymerase (5 U/µl).

Standard DNA or DNA extracted from infected cells (40 ng) was added to 10 µL of SYBR Premix Ex Taq II Kit (TaKaRa, Otsu, Shiga, Japan), 1.6 µL of a thymidine kinase primer mixture (TK 290-F: 5' TCG CGA ACA TCT ACA CCA CAC AAC; TK 400-R: 5' CGG CAT AAG GCA TGC CCA TTG TTA; each at 400 nM), 0.4 µL Rox (TaKaRa) and PCR-grade water to a final volume of 20 µl per reaction.

The primer mixture was thoroughly mixed with a brush and then applied to each tooth.