Sentence examples for primer mixing from inspiring English sources

In SSOE-PCR, the important modifications are designing shorter outermost primers (forward primer and reverse primer), mixing the outermost primer in step 2, setting higher annealing temperature in overlap extension phase and running a continuous cycling program in one reaction mixture.

Although these studies demonstrated that the number of unique tags does not necessarily need to match the number of samples, they considerably increase the complexity of sample handling by employing experimentally complicated sample or primer mixing procedures.

You don't want any of the epoxy primer mixing in with the paint you want to apply.

Of the four multiplex primer mixes, mix I was dedicated primarily to bacterial targets and a few DNA viruses.

Eight loci (from primer mixes MP2, MP3 and MP5, see Table 1) were amplified and scored for all samples.

Diluted cDNA samples were used for Real time PCR analysis with 200 nM of each primer mixed with SYBR Green PCR master as per manufacturer's instructions.

Four different primer mixes (MP2, MP3, MP5, and MP9 see Table 1) each amplifying two, three or four microsatellite loci were used.

For real time RT-PCR amplification TaqMan Universal PCR Mastermix (4304437) and primer mixes containing a FAM reporter probe (TagMan Gene Expression Assay) were obtained from Applied Biosystems.

For each method, four different DRT primer mixes (A-D) have been used.

For each of the 16 extracts, four 10-µL PCRs were conducted for each of the primer mixes in duplicate.

We performed a stepwise analysis using different primer mixes for different levels in the phylogeny (Supplementary Information, Table S2).