Sentence examples for primer optimization from inspiring English sources

Primer optimization was performed.

Sequence alignments, G+C and antigenicity profiles, and PCR primer optimization were performed using MacVector (Accelrys) software and NCBI BLAST servers.

Product dissociation curves were generated using one round of the following cycle parameters at the end of the primer optimization run: one minute at 95°C, 30 seconds at 55°C, and 30 seconds at 95°C.

Additional file 11: Primer optimization for FAD3-type genes in G. hirsutum.

If these nucleotide characteristics are taxon specific, amplification efficiency will create taxonomic biases despite primer optimization.

Further primer optimization was conducted on individuals from three host-associated populations of thrips [M, NBd and NC (Table  1)].

To test each probe for optimal efficacy, a second plate is designed for each target to enable the testing of various concentrations of each probe ranging from 25 nM to 225 nM in the presence of optimal primer concentrations (as already established by the primer-optimization plate in each case).

Probe-optimization plates were also run in the GeneAmp® 5700 sequence detection system using the same thermocycler program as used for the primer-optimization plates.

This second (probe-optimization) plate is run in the GeneAmp 5700 sequence detection system using the same thermocycler program as used for the first (primer-optimization) test plate.

On the primer-optimization plate for each target, primer amounts that, upon analysis, provide the highest Rn value with the lowest primer concentration(s) are identified as the optimal concentrations for each primer pair for each of the respective qPCR targets of interest.

Additionally, PCR primer design, optimization techniques, and contamination prevention are discussed.