Sentence examples for primer preparation from inspiring English sources

When 2N FR primers were used (lane 2), such that the primer preparation contained all 16 possible di-nucleotide sequences at their 3' ends, amplification of the exon 7 8 fragment was observed together with amplification of two additional fragments.

Primer preparations were devised in accordance with GenBank® Nucleotide Sequence Database.

The use of Transbond Plus self-etching primer for preparation of demineralised enamel significantly increased microleakage at the adhesive-bracket interface on both sides of the brackets compared to that observed with conventional acid etching or NaF treatment.

Such interaction would potentially begin the unwinding of the templated RNA primer, in preparation for its excision.

[See Additional file 4.] These new exon junctions were supported by slightly more reads from the TSEQ random primer sample preparation method (55%) and from the pooled cell line sample A (57%).

A decrease in base quality is observed towards the end of the sequence and there is a bias in nucleotide content in the first 10 cycles of the reads due to the random hexamer primer library preparation approach [ 50].

Primers for preparation of PCR products and PCR product sequencing.

The primers for preparation of PCR product and DNA sequencing are listed in Additional file 7: Table S3.

Tissue-specific targets are stored for each gene, encompassing information on primers, qPCR preparation, qPCR conditions and a publication reference.

Oligodeoxynucleotides that were used as primers for preparation of the ternary Dpo4·DNA·dNTP complexes and all unmodified template oligodeoxynucleotides were obtained from Midland Certified Reagents (Midland, TX).

Therefore, these layers must have been used as preparation, primer or corrosion protection layers.