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After the primer solution was heated to a boiling temperature, the reducing solution was then added into the primer solution.
During application of primer solution and subsequent air-drying of primer, a large portion of contained water is removed.
The duration of air-drying needed to evaporate the residual solvent from the self-etching primer solution was simultaneously measured.
The primer (solution of resinous monomers diluted in organic solvents, which in this adhesive system is presented in a separate bottle) was applied actively for 10 s.
Primer solution of Clearfil SE Protect contains an acidic 10-MDP monomer and hydrophilic components (such as HEMA) dissolved in water.
A combination between hydrophilic monomers from primer solution and hydrophobic monomers from bonding resin has produced in some cases an acidic and hydrophilic bonding agent.
Similar(28)
For the 104-loci Alu libraries, we loaded each of the 4 Primer Solutions into 12 Primer Inlets.
The peak height differences depend mainly on the relative primer concentrations and can be avoided e.g. by using premixed primer solutions containing all ARMS primers.
In brief, a total of 3 μg of genomic DNA from each sample was mixed with 40 μl of 2.5× random primer solutions.
For the 60-loci libraries we loaded each of the 12 Primer Solutions into four Primer Inlets and each of the 12 Sample Mix Solutions into four Sample Inlets.
For the 22-loci libraries, we loaded each of the 6 Primer Solutions into 8 Primer Inlets and each of the 24 Sample Mix Solutions into two Sample Inlets.
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