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In summary, the results suggest that an adequate choice of primer, primer thickness, and intumescent coating is essential for a good performance of an intumescent coating system.
A commercial primer (Primer Scotch bond multi-purpose, 3 M ESPE, St Paul, MN, USA) was applied in all groups and the solvent was dried for 5 s with an air spray.
Bobbie's #1 tip: "Primer, primer, primer!
"Primer, primer, primer," says Viviana Martin, makeup artist and director of artist relations for Kevyn Aucoin Beauty.
End sequences were obtained by Sanger sequencing with T7 Promoter Primer and BAC-R primer (primer sequences in Table S2).
And finally the Agilent primer (primer 4) having a 6 bp long 3' spacer sequence that is fully overlapping with the Ambion primer.
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For 5′ RACE, a gene-specific primer (primer-2; 5′-ATTTCCTGGGATG TCAGTGTTGGG-3′) wasynthesizeded on the basis of the nucleotide sequence obtained by 3′ RACE.
Primer-dimers are off-target amplification artefacts formed by primer-primer binding and subsequent elongation.
This step is complicated by competitive annealing between primers and incomplete fragments including primer-primer reactions.
Non-extensible dimers are primer-primer interactions which form stable structures but do not produce spurious dimer-products that elongate and amplify.
In fact, dimer prediction programs often explicitly consider the ability of primer-primer interactions to extend by reporting the most stable 3′-dimer structures.
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