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The initiation sites of these transcripts were determined by primer extension analysis.
The zinc binding domain alone can stimulate primer extension by T7 DNA polymerase.
We focus on the SNP genotyping method based on primer extension and mass-spectrometry (PEA/MS).
Protocols for RNA extraction, normalization, primer extension, and data evaluation are also provided.
The genes fdhEABCD were cotranscribed as indicated by RT-PCR and primer extension experiments.
(A) Primer extension.
The method was continuously improved and collectively called as the location specific-primer-extension principle, or the primer extension method (Onaga, 2014; Padmanabhan and Wu, 1972b).
Primer extension showed that this type 1 gene is transcribed in ovary and tadpole.
The relative mRNA levels of waaA were measured using primer extension in the wild-type Y.
A primer extension was then performed using the dATP, dCTP, dGTP, and benzyl-dUTP nucleotides.
A primer extension was then performed, and an enriched pool was prepared for the next round.
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