Sentence examples for mutations we designed from inspiring English sources

In addition, the temperature activity and pH activity profiles revealed that the mutations we designed had no obvious influence on the catalytic activity of the enzyme.

Based on individual Flt3-TKD mutations, we designed patient-specific primers to perform a highly sensitive polymerase chain reaction (PCR) assay for rapid detection of minimal residual disease (MRD).

To evaluate whether antimicrobial activity is conserved at the tryptophan rich domain (TRD) and affected by point mutations, we designed a series of peptides based on the TRD of proteins encoded by the alleles Pina-D1a, Pina-D1m, Pinb-D1a, Pinb-D1b, Pinb-D1l and Pinb-D1q, as well as by the grain softness protein-1, hordoindoline-a and hordoindoline-b genes.

For RNA genes' mutations, we designed a bioinformatic tool which compiled evolutionary conservation and potential effect on RNA structure.

To challenge MotorPlex to be applied to large studies on thousands of patients and/or to detect mosaic mutations, we designed a combinatorial pooling strategy.

To introduce the desired point mutations, we designed and used the following primers: Neo-Bcl-2 K/D forward (5′-GACAACCGGGAGATAGTGATGGACTACATCCATTATAAGCTGTCG-3′); Neo-Bcl-2 K/D reverse (5′-CGACAGCTTATAATGGATGTAGTCCATCACTATCTCCCGGTTGTC-3′).

In order to identify the causative mutation we designed a custom 1× tiling bait array to capture a targeted region encompassing this region.

To search for the causative mutation, we designed oligonucleotide primer sequences based on the genomic sequence of Amelx (ENSMUSG00000031354) and sequenced each exon and the associated splice junctions using dye primer chemistry.

To create point mutation constructs, we designed primers containing point mutation(s) and performed standard site-directed mutagenesis methods with minor modifications.

To detect both mutant and wild-type mtDNA, even when polymorphisms were present near the target mutation sites, we designed specific oligonucleotide probes.

To increase the mutation efficiency, we designed gRNA vectors containing one to three gRNAs directed against different regions of the same gene.