Sentence examples for mutations we developed from inspiring English sources

Although several methods exist to detect these targeted mutations, we developed a heteroduplex mobility assay on an automated microfluidic capillary electrophoresis system named HMA-CE in order to accelerate the genotyping process.

For the rapid and sensitive screening of epidermal growth factor receptor (EGFR) hot-spot mutations, we developed a novel method combining mutant-enriched PCR with amplification refractory mutation system (ARMS) TaqMan real-time PCR in a one-step reaction tube.

To detect CD20 in the clinical specimens that acquired rituximab resistance mutations, we developed novel anti-CD20 antibodies and screened them in paraffin sections.

To facilitate the analyses of cancer mutations, we developed the second version of the Roche Cancer Genome Database (RCGDB) [ 14], an integrative biological information system combining different databases which are already publicly available.

To screen for these mutations, we developed an in vitro nonradioactive assay based on phosphorylation of a biotinylated ROCK2 substrate peptide matching the consensus ROCK2 phosphorylation site in LIMK.

Based on the framework of the modified Tajima's D test for DNA mutations, we developed a neutrality test with the statistic " Dm" to detect selection forces on DNA methylation mutations using single methylation polymorphisms.

In order to first establish the physiological relevance of Flt3-ITD as an AML-inducing mutation, we developed a mouse model in which Flt3-ITD was coexpressed with an inducible deletion of the DNA-binding domain of Runx1 (Growney et al., 2005) in MPPs.

To detect CD20 with these mutations comprehensively, we developed monoclonal antibodies that recognize a part of the amino-acid sequence of the N-terminal cytoplasmic region of CD20.

Considering that the extensive study of the CFTR gene classically performed in molecular diagnosis does not detect all disease-causing mutations, we previously developed an approach for a complete resequencing of the CFTR locus to search for mutations deeply located in introns (Bonini et al. Genet Med, 2015).

To better facilitate the appreciation of and interaction with these data and to compare the effects of specific genes in the context of different polymerase mutations, we have developed an online data visualization tool called DIXY (http://bsu-srv.ncl.ac.uk/dixy-pol).

To assess the frequency and extent of EGFRvIII mutations in GBM, we developed two independent methods for quantitative measurement of EGFRvIII.