Sentence examples for mutations we generated from inspiring English sources

As the six mutants contained multiple mutations, we generated enzymes with only single-point mutations, expressed them in E. coli, and determined their activity.

To investigate the biological effect of ALS-causing genetic mutations, we generated motor neurons from SOD1 +/A272C iPSCs and corresponding isogenic controls using a modified protocol with high differentiation efficiency (Qu et al., 2014; Maury et al., 2015) (Fig. 3A).

Instead of generating p53 germline mutations, we generated mice that allow global somatic deletion of one or both copies p53 at any chosen time.

To measure the fitness of HIV-1 RT mutants along an AZT resistance pathway and to evaluate the effect of epistasis ( =  the fitness interactions between the mutations), we generated the respective RT mutants by site-directed mutagenesis PCR [51].

The top shows truncating mutations, while the bottom shows the missense mutations we generated.

Based on the two effective single mutations, we generated an AQP1 mutant containing the double mutation D237A and K243C (Fig. 6).

For each such BTP and set of mutations, we generate a VAF data corresponding to each tumor subpopulation.

To begin to address the specific effects of this commonly observed cTnT mutation we generated two independent transgenic mouse lines carrying variant doses of the mutant transgene.

To identify smg11 mutation, we generated an F2 population from a cross between smg11 and KYJ.

To further examine the molecular consequences of the ingls Vac14 L156R mutation, we generated the equivalent yeast mutant, vac14 L149R.

Finally, to assess if this cooperation was restricted to H-Ras mutation, we generated a cohort UroIICRE+ β-catenin exon3/exon3 K-Ras G12D (n=25).