To exclude the possibility of either polar or suppressor mutations in the mutant, we constructed complementation strains with wt pppA-ppkA genes expressed ectopically.
To better understand functional interplay between trisomy 21 and GATA1 mutations in hematopoiesis, we constructed cellular disease models using human induced pluripotent stem cells (iPSCs) and genome-editing technologies.
To directly test the hypothesis that the frequency of incorporating an SSG mutation depends on the distance of this mutation from URA3, we constructed a plasmid (pS660) carrying a 750 bp region of the IME1 promoter that contained 9 mutations (Fig. 3A, i).
To model homozygous mutation disease embryos, we constructed nuclear transfer embryos by fusing the lymphocyte or skin fibroblast cells with enucleated in vitro matured (IVM) oocytes.
Here, to provide a higher order view of the transcriptional changes caused by the TS mutation in CACNA1C, we constructed genome-wide transcriptome networks in control and TS neural progenitors and differentiated neurons at rest and following depolarization.
To reveal connections between DNA-protein interactions and expression in mutations of the PRE, we constructed seven plasmids that mutated the PRE sequence identically to the mutant PRE oligomers.
Given that mutations in MEI-S332 that inhibit phosphorylation by Aurora B reduce centromere localization both in cell culture mitosis and in meiosis, we constructed mutations that cause phosphomimetic amino acid substitutions at the three serines required for Aurora B phosphorylation.
To better study the impact of rpoB mutation on meningococcal virulence we constructed an isogenic mutant strain, NM05-08, by transforming the susceptible isolate LNP21362 with a PCR-amplified fragment from a resistant isolate (LNP22330), as previously described (6 ).
We constructed mutations in all three of these conserved domains to assess their function in S. pombe.
To determine the significance of the interaction between Edc3 and NAD(H), we constructed mutations in the putative binding pocket on the hEdc3 YjeF_N domain that would be predicted to alter the binding of Edc3 to NAD(H).
