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By isolating individual mutations, we identified specific amino acid changes that dramatically improved discrimination.
However, the two inactivating missense mutations we identified are very common and present in almost 100% of individuals, which makes these the most likely and sufficient causes of Forssman antigen negativity in humans.
From a collection including more than 5,000 lethal mutations, we identified 133 mutations representing ∼70 or more genes that subdivide the tracheal terminal branching program into six genetically separable steps, a previously established cell specification step plus five major morphogenesis and maturation steps: branching, growth, tubulogenesis, gas-filling, and maintenance.
Therefore, the two mutations we identified in NEXN may further increase our knowledge of Z-disc genes in the pathogenesis of LVNC.
The mutations we identified need to be tested for their effect on transmission in other A/H5N1 virus lineages (60), and experiments are needed to quantify how they affect viral fitness and virulence in birds and mammals.
The specific mutations we identified in these experiments that are associated with airborne transmission represent biological traits that may be determined by a set of different amino acid substitutions.
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The pfatp4 mutations we identify localize to the transmembrane domain suggesting inhibitors bind in the clefts within transmembrane helices.
We also now highlight that the individual deleterious mutations we identify will provide an invaluable platform for the mechanistic dissection of buffering in the future.
If any of the 7 mutations we identify above are contributing to the phenotype, they are doing so in a background that excludes the e14 prophage.
Summarizing the data from the literature of homozygous severely obese subjects with a homozygous LEP mutation, we identified 53 subjects with 12 distinct variants in the leptin gene.
Strikingly, this PDX did contain the MEK1 T55delinsRT mutation we identified in M032R4.
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separations we identified
transfers we identified
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mutant we identified
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mutations we hypothesized
mutations we examined
mutations we generated
mutations we sequenced
mutations we studied
mutations we investigated
mutations we considered
mutations we analysed
mutations we screened
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