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This study was spurred by our identification of a newly identified recessive mutation that suppresses the SI phenotype of SRK-SCR transformants of the Col-0 accession.
Here, we use a newly identified induced mutation that suppresses the SI phenotype in stigmas of SRK-SCR transformants of the Col-0 accession to investigate the regulation of SI and the SRK transgene.
Further, we identify a second-site suppressor mutation that suppresses some but not all of the phenotypes of tpp1− cells, suggesting that the perturbation of second-site gene products could provide a therapeutic approach for NCL.
This lack of effect on the catalytic activity for a mutation that suppresses the conformational broadening behaviour for much of the active site appears to be inconsistent with the earlier NMR relaxation analysis that ascribed this line-broadening to a sampling of the transition state conformation by the unligated enzyme [ 14].
E. coli groEL3388, as well as the other three strains, carries the original groEL44 (E191G) mutation and an additional intragenic mutation that suppresses the temperature-sensitive phenotype of groEL44 bacteria, allowing them to form colonies at 43° (Klein and Georgopoulos 2001).
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As pla1-10, the original insertion mutation that suppressed sme2Δ, did not seem to retard vegetative growth, a defect in the polyadenylation complex might have a more significant impact on meiotic transcripts than on mitotic transcripts.
Since, prior to this study, only five Su TPE) loci were known and we had only been able to identify two additional Su TPE) from our analysis of Su var)s and two from our analysis of the Su z 25 deletion, we undertook a screen designed to identify mutations that suppressed TPE.
Long-lived dwarf mouse strains carry mutations that suppress the growth hormone (GH /insulin-like GH /insulin-like(Igrowthendocrine pathway [ 1].
It will be very interesting to determine whether mutations that suppress the adverse effects of aneuploidy also delay aging and extend the span and quality of life.
To identify genes acting with IAR1 to control conjugate sensitivity, we conducted a genetic modifier screen to isolate extragenic mutations that suppressed the iar1 mutant phenotype.
Ninety-seven percent of mutations that suppressed mot1-301 expression were in genes that encode components of the SUMO pathway (Wang et al. 2006), and mutations in every step of the SUMO pathway suppressed mot1-301.
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mutation that disrupts
mutation that stops
mutation that destroys
mutation that precludes
mutation that eliminates
mutation that reduces
mutation that removes
mutant that suppresses
mutation that reduced
mutation that prevents
mutation that restricts
mutation that has
mutation that guarantees
mutation that lets
mutation that interferes
mutation that closes
mutation that does
mutation that is
mutation that arises
mutation that lowers
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