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The observation that∼50% of the conditional mutants we analyzed are potentially fast-acting provides additional incentive to isolate more TS alleles.
Background/Aims: Hepatitis B virus mutants of the polymerase gene are frequently selected during lamivudine therapy for chronic hepatitis B. To study the biology of these mutants, we analyzed their replication capacity in the duck hepatitis B virus (DHBV) infection.
Most of the dumpy mutants we analyzed were generated by EMS following Jenkins [13] in stocks isoallelic for dumpy.
The number of IMAs increased by 138%, which is the highest value amongst the mutants we analyzed (Figure 3A and 3B).
To confirm known morphology defects of mutants, we analyzed the fungal morphology in the CAM by histology 24 h, 48 h, and 72 h p.i.
To determine the effect on Muc16/MUC16 expression in homozygous mutants, we analyzed the expression of Muc16 mRNA and protein by RT-PCR and immunofluorescence, respectively.
Similar(27)
We chose to focus further genetic characterization on the only miRNA mutant we analyzed that showed a severe lifespan phenotype (mir-71).
To test this idea for the ado1∆ mutant, we analyzed the effect of this mutation on ADE4 expression and found it to be reduced at day 8, consistent with such a model.
To evaluate the specific elimination of mutant mtDNA in patient-derived iPSCs and the potential use of mitoTALENs to prevent the germline transmission of mutant mtDNA, we analyzed the ability of mitoTALENs to reduce mutant mtDNA levels in mammalian oocytes.
To determine if this is also the case in DLST-deficient ste−/− mutant zebrafish, we analyzed the mitochondrial ultrastructure in ventricular and atrial cardiomyocytes of ste−/− mutants by transmission electron microscopy (TEM).
Because of the lethality of C57/BL6J backcrossed null mutant mice, we analyzed heterozygous Scrapper gene-deficient mice [SCR ] using behavioral test battery.
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changes we analyzed
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