To verify whether the retroviral KLF4 and OCT3/4 transgenes were present in our iPS clones, we performed PCR analysis and observed both transgenes were integrated in all clones we analyzed (Figure S1A).
In the clones we analyzed, amplification of the tag via rolling circle replication would have required the formation of a circular molecule encompassing the entire plasmid sequence (∼7 kb excluding the (TTAGGG n arms).
Among the 52 positive clones we analyzed, 39 encoded B group proteins (i.e., B-3 subfamily members), whereas 13 encoded A group proteins (i.e., A-6 subfamily members) (Table 1).
To facilitate understanding the pathogenesis of this hypervirulent clone, we analyzed a clinical isolate (IA3902) of clone SA using multi-omics approaches.
To exclude that the miR expression profile was peculiar of the 21-5 clone, we analyzed the expression level of the 16 miRs in two other HCV replicon clones, 22-6 and 21-7.
Using the cDNA microarrays spotted with duplicated 7,334 cDNA clone, we analyzed RNA specimens successfully amplified in 44 peripheral blood collected from 25 confirmed SARS patients (age ranged from 23 to 80 years old, mean = 41.8, SD = 17.2, median = 34), of whom 24 survived.
In parallel to analyzing Pcl mutant clones, we also analyzed wing discs with clones of cells that were homozygous for null mutations in E z) or Su z 12, respectively.
To cross-validate our cloned data, we analyzed genomic DNA from ρ0 cells, blood, and human placenta using mitochondrial primers that co-amplified nuclear loci in the prostate cancer specimens.
Therefore f, the frequency of mwh+ Y chromosome loss, is f = n 2 m/ N C. To determine the spontaneous frequency of mwh clone formation, we analyzed wings of (1) w / mwh + Y; mwh males that developed (i) on standard Drosophila food or (ii) on the 4-24 instant Drosophila medium and (2) wings of w / mwh + Y; mwh lds hor-rvP2/ mwh lds+ males.
Given the observed up-regulation of DKK1 in cisplatin-refractory NSCLC residual clones, we next analyzed basal mRNA DKK1 expression levels in NSCLC cell lines with the aim to reveal if there was a correlation between basal DKK1 expression level and cisplatin sensitivity (Fig. 4c).
To determine if human NPM3 is similarly located and to identify genomic clones of NPM3, we analyzed FGF8-containing human genomic lambda clones [ 22] by Southern blotting using a mouse Npm3 cDNA probe.
