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Of a number of well-characterized mutants, we selected three: R50A, N51A and R190X (Table 1).
Out of 20 mutants, we selected 3 clones exhibiting relatively high affinities to nucleolin on yeasts.
Among these mutants, we selected the one that exhibited the highest organic solvent tolerance and investigated the contributions of each identified mutation to organic solvent tolerance.
From those mutants, we selected 64 strains of which the affected locus was located in a coding region.
To screen for the desired Cln3 mutants, we selected 12 single amino acid sites in clustered charged residues (Miller et al., 2005).
To examine whether the improved integration efficiency of PlasmoGEM vectors would permit the reproducible generation and phenotyping of mixed pools of barcoded mutants, we selected from the resource 46 vectors targeting protein kinases that had previously been part of a systematic deletion analysis using conventional vectors (Tewari et al., 2010).
Similar(54)
Among the many Calvin cycle enzymes found to be affected in the ΔmcyB mutant, we selected RbcL for a more detailed study.
The first apoc2 mutant we selected had a frame shift mutation, which led to the amino acid sequence change in its Lpl-binding domain (Fig. 1B,E).
We observed 5% or less embryonic lethality in 20 mutants that we selected for further analysis, reasoning that most of these mutants likely carry recessive loss-of-function mutations, with embryonic lethality at levels similar to that observed when wild-type worms were up-shifted to 26° as L4 larvae.
In contrast, we identified over 100 mutants when we selected for outgrowth of clones in the soft agar colony-forming assay.
In addition to a previously established LSE1 mutant line, we selected two other independent lines, which exhibited distinct starch accumulation in leaf blades of 4-week-old seedlings, in this study.
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