Sentence examples for mutants we constructed from inspiring English sources

Thus, in this study, we isolated suppressor mutants of rrn5 disruption and by utilizing these mutants we constructed a yeast strain exhibiting high RNA content probably due to increased transcription of NTS region which is normally silenced.

We therefore sought to determine whether the mutants we constructed were functional.

To analyze F-spores derived from germination-defective mutants, we constructed F-spores from two different mutants of B. subtilis, FB72 (ger-A ger-B gerK) and FB113 (cwlJ sleB).

We also tested if DNA methylation accumulated in any of the dmtA/tmdA mutants we constructed, and found that in both asexual and sexual tissues, these mutants, just like wild-type strains, appear devoid of DNA methylation.

All R. sphaeroides mutants we constructed contained in-frame markerless deletions.

The mutants we constructed will be useful for investigating whether the different secondary scaffoldins have specialized roles in binding enzymes needed to degrade substrates other than Avicel.

To exclude the possibility of either polar or suppressor mutations in the mutant, we constructed complementation strains with wt pppA-ppkA genes expressed ectopically.

To test this possibility with the pol2-Y831A mutant, we constructed haploid yeast strains with either single or double mutations in the POL2 gene, one in the polymerase active site pol2-Y831Aanotheranother in the exonuclease active site (pol2-4).

As CaZF could suppress osmosensitivity of hog1 and cnb mutants separately, we constructed a double mutant hog1cnb to test the functional ability of CaZF in absence of both Hog1p and Calcineurin.

The mutants that we constructed are easily cured of their tetRA cassette to produce markerless mutants.

Among the 23 mutants that we constructed, the most promising Y216W showed an 18 ± 2.7% increase in specific activity by comparison with the wild type enzyme.