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Similar phenotypes were obtained crossing Dcas1/Dcas1 to two additional Src42A mutant strains including the mild allele Src42Amiri and the strong allele Src42AE1 (Table 1 and not shown).
Of note, unlike other respiration deficient mutant strains (including rho0 and Δmgm1) the Δoxa1 strain showed same growth rates as the wild-type strain in liquid cultures and during colony growth, respectively (Fig. 2A and B).
Several expression microarray experiments based on the wild-type strain and mutant strains including both overproducing and defected strains were later on released [ 6, 28- 30].
The integrated strain, carrying 99 CGG repeats in the 5′UTR of the GFP reporter, was crossed with mutant strains, including alg-2 ok304 alg-2 ok3041), hpl-2(tm1489) and mut-7(pk204).
The S. pombe haploid, wild-type strain 972 h− and its derivative mutant strains, including the temperature-sensitive (ts) cut14-208 (Saka et al. 1994) and cold-sensitive (cs) nda3-KM311 (Hiraoka et al. 1984), were used.
S. pombe deletion mutant strains, including fkh1Δ, tco89Δ and tor1Δ deletion mutants, were obtained from the Bioneer version 2.0 (Bioneer Corporation, Daejeon, Korea) library and PCR-verified for correct deletions at both junctions.
Similar(53)
Mutant strains included sta1-1 (I7), sta6-2 (Band3), sta6 (BafJ5) and sta7-1 (BafJ6).
In this study we investigated the phenotypic properties of a bba64 mutant strain, including 1) replication during tick engorgement, 2) migration into the nymphal salivary glands, 3) host transmission, and 4) susceptibility to the MyD88-dependent innate immune response.
To do so, we analyzed the gene expression of several chaperones in the atx-3 mutant strain, including three small heat shock proteins (sHSP) HSP-16.49 HSP-16.491, HSP-16.1) and four members of the HSP70 family (F44E5.5, HSP-16.2SP-1, C12C8.1), using real-time PCR.
The expression of several genes involved in sulphur metabolism is upregulated in the mutant strain, including sulphate binding proteins Sbp and CysP, sulphite reductase CysI, and the ATP sulphurylase, CysND.
These mutants strains include those defective in nucleotide excision repair (rad2), postreplication repair (rad18, rad27a, and ubc13), base excision repair (apn1, rad27, and ntg1), homologous recombination (rad52), DNA mismatch repair (mlh1), and DNA damage bypass (rad30), glutathione synthesis (gsh1 and gsh2), oxidative stress (sod1, sod2, and cta1), and protein disaggregation (hsp104).
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