Sentence examples for mutant strains contained from inspiring English sources

The Δpvl mutant strains contained a spectinomycin resistance cassette (spc) used to replace pvl genes, which allowed for the enumeration of the Δpvl mutant (Spc-resistant) and parent strain (Spc-sensitive).

Less than 20% of the cells of the wild-type, recB, and recG mutant strains contained foci, and only 1 2% of the cells of the ruvA mutant and ruvA recG double-mutant strains contained foci.

Consistent with this idea, aggregates from both mutant strains contained many constituents of multisubunit assemblies, and the species we detected only in aggregates from ncs2Δelp6Δ yeast were mostly additional subunits of complexes aggregating in both mutants (Table S3).

It may be noted that all mutant strains contained the βY331W mutation, which is valuable for measurement of nucleotide binding parameters and does not affect function significantly on its own.

Images of cells in exponential phase revealed that greater than 90% of the cells of the wild-type, recG, recB, and ruvA mutant strains contained foci, whereas only 74% of the cells of the ruvA recG double-mutant strain contained foci (Table 2 and Figure S6).

These results were confirmed by the phase-contrast images in Figures 3– 7 and Figure S1, Figure S2, Figure S3, Figure S4, and Figure S5, which show that the morphology of the wild-type strain was constant throughout the course of the experiment but that the cultures of the mutant strains contained both normal and filamenting cells.

Others have examined mutant strains containing deletions in outermembrane proteins that show biofilm formation in the animal pathogen B. bronchiseptica is protein-mediated [18].

Until recently, it has proven difficult to construct isogenic L. monocytogenes prfA* mutant strains containing the alleles that confer the highest PrfA activity by standard methods.

These mutant strains contain a kanamycin marker in SGI1 that was necessary for the selection of SGI1 in mating experiments according to the large antibiotic resistance profile of these plasmids (Table 1).

This mutagenesis procedure resulted in the creation of S. aureus single mutant strains containing a deletion of htsA (ΔhtsA) or a replacement of isdE with an erythromycin resistance marker (ΔisdE).

In addition, in a mouse model of Lyme disease, B. burgdorferi mutant strains containing bbk32 gene deletions have an attenuated infectivity when assessed at twenty-one days post infection [39], demonstrating that BBK32 is a virulence factor in borrelioses.