This effect was specific: it was not seen in vivo with knock-down of the other CDKs and was not seen in other mutant KRAS expressing tissues including the pancreas.
Making use of two FLP lines, T155-Gal4, UAS-FLP (hereferredreferred to as T155-FLP) and Hsp70-FLP, we are able to generate Smr mutant clones in various tissues including the wings, legs, and ovaries (the activity of T155 in the imaginal discs and ovary are shown in supplementary material Fig. S1A C).
PTRN-1 GFP PTRN-1 GFProm its expresseds promoter (P ptrn-1) rescued the synthetic lethality ofrome noca-1∆; ptrn-1∆ double mutant and localitsd in multiplendogenous including the larval/adult epromoter, neurons, intestine, and Pharynx.
Yet these two XX mutants have morphologically indistinguishable somatic tissues, including the somatic gonad (Barton et al. 1987; Schedl and Kimble 1988).
The progression of autophagy in the mutant mice may be only partially blocked, since mutant fibroblasts contain residual PI 3,5 P2 and many tissues, including liver, do not exhibit degeneration (2).
In the mutant, we observed abnormal ciliogenesis in multiple tissues, including in the embryonic node, neural tube, kidney and cochlea of the inner ear.
These Fhit mutant mice develop tumours in a variety of tissues, including intestinal adenomatous polyps, and show abnormal tissue building of intestinal mucosa, suggesting avenues for further study of Fhit function in normal tissues.
Transgenic mutant embryos derived from this mutant ES cell clone exhibited functionally significant mtDNA depletion in multiple tissues, including brain and muscle, as well as elevations in MMA levels.
Similarly, recent research in B. distachyon indicated that CAD mutants displayed the red-brown pigmentation in various tissues including nodes and flowers, but not in the leaf midrib [ 64].
We have used microarrays for expression profiling of wild-type and Foxa2 mutant embryos at E7.5, which fail to form organizer-derived tissues, including the node, AME, and DE.
