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Several independent AtCRT1a-transformed Atcrt1b mutant lines displayed an increase in AtCRT1a mRNA production and AtCRT1a protein accumulation (Figures 8E and F).
However, only one of these mutant lines displayed a near-complete reversion to the wild-type phenotype.
Whereas wild-type Arabidopsis thaliana of the Columbia ecotype possess telomeres with lengths ranging from 2 to 5 kb, mutant lines displayed remarkable shortening.
Both rps19 mutant lines displayed a similar phenotype and we chose the first mutant line (noted as 'Mu1' in Figure 1c) for further investigation.
As trt-1 mutants approached sterility we noticed that two of the trt-1/ceob2 mutant lines displayed high variability in the amount of progeny having plates that looked like trt-1 mutants and others with larger brood sizes.
Similar(55)
In addition to smaller grain size and panicle height, average single grain weight was also lower in the mutant lines, displaying an absolute recovery for the trait in C4 (Fig. 1i).
Dose-dependent sensitivity to Nutlin-3 is displayed by both wild-type lines UM-SCC-17A and UM-SCC-17AS, whereas all of the mutant lines display essentially identical growth in the presence and absence of Nutlin-3.
Yet aside from the generation of biological and methodological puzzles that invite further study, the present investigation also yielded intriguing mutant lines displaying a remarkable spectrum of leaf morphology alterations and resulting from manipulations of previously uncharacterized genes, thereby opening new avenues to the characterization of novel gene functions.
However, towards the end of the sterility assay this trt-1 mutant line displayed strong developmental defects and had a very small brood size, whereas all wt controls and the DM survivors were still reproductively active.
For example, by mating the lox-eGFP-lox-DTA mice with the Pax3-Cre mice, the mutant line displayed variable perinatal lethality rates with a lower number of astrocytes in the dorsal spinal cord, but without an increase in their BBB permeability.
Neither the WRKY25 overexpression lines nor the mutants lines displayed altered fungal growth or symptom development (unpublished results).
Related(17)
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