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The enzyme activity in the mature leaves of the mutant lines was lower by 28.8% relative to the WT, suggesting a reduction in soluble acid invertase activity in the mutants, with observed recovery in the C4 line (Fig. 1e).
Accession numbers of the Atcyn mutant lines was listed in Table 4.
The original CyO balancer of the sumo mutant lines was replaced with a GFP expressing CyO balancer (CyO, ActGFP) to allow homozygous SUMO mutant flies (which lack GFP) to be distinguished from their heterozygous siblings.
In order to address this problem in the present study, the DNA content of the mutant lines was determined by FACS analysis and compared to that of the wild type.
A subset of 768 mutant lines was analyzed by TILLING using four target genes.
Pituitary tumor development in all three mutant lines was associated with mouse chromosome 14.
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The homozygous overexpressor and knockout mutant lines were confirmed using western blot analysis.
The novel mutant lines are useful genetic resources for production or future cowpea breeding programs in Namibia or similar environments.
Amylose content, seed morphology, and starch granules of pul mutant lines were essentially the same as those of wild-type plants.
The panicles from WT and mutant lines were harvested post-maturity (40 DAH) and air-dried for 2 weeks before analyses.
Mutant lines were maintained in the AB* and WIK backgrounds.
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