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Most of the mutant lines exhibited elevations in the fatty acid species that serves as a precursor to the mutated step.
Furthermore, mutant lines exhibited a wide range of developmental abnormalities affecting flowers and siliques, premature death of seedlings, reduced male & female fertility, and embryonic defects [ 3, 4, 8].
Triple and quadruple mutant lines exhibited dwarf rosette growth, reduced plant height, late flowering phenotype, reduced fertility and also reduced growth of roots and root hairs.
The apparent amylose peak from the Atss2 mutant lines exhibited a maximal absorbance wavelength when complexes with iodine (λmax) of approximately 610 nm, closely matching the wild type value.
When we probed western blots for COL4A1 levels (or COL4A2 levels in the case of Col4a2 +/G646D), we found that, compared with samples from Col4a1 +/+ littermates that were run on the same gel, all mutant lines exhibited significantly increased levels of intracellular COL4A1 (or COL4A2 in the case of Col4a2 +/G646D) with the exceptions of Col4a1 +/G394V and Col4a1 +/G658D (Fig. 5B).
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Atbg1 mutant lines exhibit lower ABA level and ABA defective phenotypes whereas over-expression lines increase levels of dehydration- induced ABA accumulation.
All mutant lines exhibit aberrant regulation of bradyzoite gene expression and notably some of the mutant lines appear to exhibit high proportions of the intracellular tachyzoite state regardless of whether they are intracellular or extracellular.
It has been reported that the 05342 and 08941 mutant lines exhibit a decrease in Tn I transcript levels in embryos, pupae, and adults [14], which appears to be in contradiction with our results.
Mutant lines exhibiting very short seed fibers include the Ligon Lintless-1 and −2 lines (Li 1 and Li 2 ) [ 18, 19].
In agreement with findings by Murakami et al. [ 31], the fad7 fad8 mutant line exhibited the most pronounced tolerance to high temperature based on growth rate.
At the same time, all four cerebellar mutant mouse lines exhibited poor interlimb coordination.
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