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Note that although some mutant variant lines displayed clearly visible truncated C-terminal fragments (∼25 kDa) that have been previously shown to be the result of caspase activation (reviewed by Baloh, 2011), some lines only showed a modest or barely detectable truncation (Fig. 1Y).
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Mean fluorescence intensity (MFI) measurements of C400 in UCP2-KD variants of these cell lines displayed a significant increase in ROS levels (2 to 3.5-fold, Figure 4a).
None of the lines displayed high sensitivity (Figure S2).
By contrast, R1507-resistant cell lines displayed different signaling properties.
CD146+ cell lines displayed a mesenchymal phenotype.
Five GUS-positive lines displayed N-responsive GUS activity.
The intermediate DU145-LN cell lines displayed intermediate phenotypes.
The cell lines displayed different sensitivity to the anticancer drugs.
TUBO-P2J and TUBO cell lines displayed a different morphology.
None of the cerebellar mouse lines displayed deficits in motivation.
Both cell lines displayed modest sensitivity to TNF.
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