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These isogenic WT and mutant lines were differentiated to D30_L using our established strategy (Fig. 1a).
Stat3 MuSCs from both mutant lines were defective in proliferation.
The homozygous overexpressor and knockout mutant lines were confirmed using western blot analysis.
Amylose content, seed morphology, and starch granules of pul mutant lines were essentially the same as those of wild-type plants.
The panicles from WT and mutant lines were harvested post-maturity (40 DAH) and air-dried for 2 weeks before analyses.
Mutant lines were maintained in the AB* and WIK backgrounds.
The dcl2 and dcl3 mutant lines were null mutants generated by T-DNA insertion, whereas the dcl1 mutant lines were partial loss of function mutants.
Mutant lines were obtained from the SALK [61], and the Versailles mutant collections.
Tüebingen wild-type lines and axintm213 (previously known as masterblind (mbl)) mutant lines were used.
Seven of the 10 EGFR mutant lines were sensitive at a clinically achievable concentration (<1 µM).
The dcl1 mutant lines were sterile and could not be tested.
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