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By the 5th day, some intolerant mutant lines began to die, while the tolerant lines by the 11th day.
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To determine the molecular basis of ovotestis and ovary development in these mutant lines we began by carefully quantifying expression of Sox9 during the sex-determining period of gonadogenesis, ∼11.25 12.5 dpc, using qRT-PCR.
Focus on where lines begin and end.
The El Tiante line began shipping in October.
One BRT line began operating in June.
Commercialization of this product line began in the 1950s.
Jeng et al. (2012) discovered mutant lines that have higher Fe or Zn concentrations in polished seeds by searching among NaN3-induced mutant lines (Oryza sativa cv. IR64).
Such differences between wild type and mutant have been discovered efficiently in recent years by using NGS sequencing of bulked DNA of mutant F2 progeny from crosses between mutant lines and parental lines in several plant and animal systems.
Instead, most groups focus on the characterization of specific mutant lines.
Consistent with this observation, Hd3a mRNA accumulation is lower in se5 hd1 than in se5 mutant lines.
Requesting the mutant lines involves a complicated process such as MTA and quarantine.
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