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The MM55K mouse kidney cell line was purchased from American Type Culture Collection (ATCC, Manassas, U. S. A). and cultured with high-glucose DMEM containing 10% fetal bovine serum and 50 U/ml Penn-Strep.
In order to select the most relevant set of molecules, we conducted comparisons between the potent PA6 cell line and mouse embryonic fibroblasts (MEF), a mouse kidney cell line MM55K, and subtypes of PA6 and MS5 lines that lack DA-inducing activity.
To identify GBPs, we used mouse kidney cell lines as described previously.
Subsequently, two additional serine proteases activating ENaC, mCAP2 (homologue of human transmembrane protease serine 4, TMPRSS4) and mCAP3 (MT-SP1/matriptase or epithin), have been cloned from the mpkCCDc14 mouse kidney cell line (Vuagniaux et al, 2002).
In addition, Bak (and to a lesser extent Bax) seems necessary for the long-chain ceramide generation during baby mouse kidney cell apoptosis induced by UV-C radiation or cisplatin treatment.
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The mouse kidney cells (TMCK-1) were a gift from Dr. T.J. Lee (Yeungnam University, Korea).
Administration of HGF partially reverses TGF-β-induced EMT in mouse kidney cells [ 31, 32].
Total RNA was isolated in Trizol (Invitrogen, Groningen, Netherlands) from samples and mouse kidney cells.
These results confirmed the ability of both drugs to inhibit efficiently the CFTR-mediated Cl− currents in mouse kidney cells.
Mitochondria from bax−/− bak−/− BMK (baby mouse kidney) cells (either expressing Smac-mCherry or not) were isolated as described.
In contrast, the presence of DSBs without γ-H2AX foci formation has been demonstrated in mouse kidney cells when bathed in high salt [ 42].
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