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They are histologically regarded as a normal finding in the mouse kidney tissue.
Right mouse kidney tissue samples were fixed in 4% formalin and processed to paraffin blocks.
g2 – Z. Gatalica, MG-U74Av2, mouse kidney tissue.
Paraffin-embedded sections of mouse kidney tissue and human colon carcinoma were used as positive controls for p-Akt1/2/3 and CIP2A, respectively, as described in the datasheet from the manufacturer.
Nephronectin was also found to be expressed in mouse kidney tissue in a pattern that matched that of α8β1, and mice lacking nephronectin showed similar phenotypes to mice lacking the α8 subunit.
Paraffin-embedded sections of mouse kidney tissue and human colon carcinoma were used as positive controls for p-Akt1/2/3 and CIP-2A, respectively, as described in the datasheet from the manufacturer.
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Bio-imaging experiments revealed that L1 and L2 could qualitatively monitor Hg2+ or Cu2+ in Hela cells and mouse kidney tissues.
The remaining halves of the harvested tumor tissues were snap-frozen for protein analysis, from which mouse kidney tissues were surgically removed using a dissecting microscope prior to freezing.
We then immunostained normal mouse kidney tissues with multiple markers (data not shown) and then applied the system to immunostain human renal tumors.
Mouse kidney cortex tissue was homogenized in a buffer consisting of 20 mM Tris (pH 7.4/HCl), 5 mM MgCl2, 5 mM NaH2PO4, 1 mM ethylenediamine tetraacetic acid (pH 8.0/NaOH), 80 mM sucrose, 1 mM phenyl-methylsulfonyl fluoride, 10 μg/ml leupeptin and 10 μg/ml pepstatin, and subsequently centrifuged for 15 min at 4,000 g.
These results are in agreement with a previous study on gene expression levels in mouse brain and kidney tissue where an inflammatory response was not triggered 8 and 24 h after irradiation [15].
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