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Tissues extracted from the human and mouse kidney cortex samples were washed with PBS and resuspended in 100 µl lysis buffer (50 mM Tris-HCl pH 8, 0.1 mM EDTA, 0.5% Triton X-100, 12.5 mM β-mercaptoethanol) for 30 min on ice.
In a normal mouse kidney cortex, C24, C22, and C16 ceramides have been identified [ 34].
In our hands, the antibody stained the tight junctions of the thick ascending limb of Henle on rat kidney sections, as previously described in mouse kidney cortex (Angelow et al. 2007).
A technique was developed to study the invasion of cells into mouse kidney cortex in the presence of crude rat liver histone at a medium concentration of 100 μg./ml./ml
Mouse kidney cortex tissue was homogenized in a buffer consisting of 20 mM Tris (pH 7.4/HCl), 5 mM MgCl2, 5 mM NaH2PO4, 1 mM ethylenediamine tetraacetic acid (pH 8.0/NaOH), 80 mM sucrose, 1 mM phenyl-methylsulfonyl fluoride, 10 μg/ml leupeptin and 10 μg/ml pepstatin, and subsequently centrifuged for 15 min at 4,000 g.
Mouse kidney cortex was homogenized in a homogenizing buffer [20 mM Tris (pH 7.4/HCl), 5 mM MgCl2, 5 mM NaH2PO4, 1 mM ethylenediaminetetraacetic acid (pH 8.0/NaOH), 80 mM sucrose, 1 mM phenyl-methylsulfonyl fluoride, 10 μg/ml leupeptin, and 10 μg/ml pepstatin] and subsequently centrifuged for 15 min at 4,000 g.
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The aim of this study was to evaluate the impact that time of day may have on 131I-induced effects in the mouse thyroid, kidney cortex and medulla, liver, lungs, and spleen.
In this study, we analyzed genome-wide transcriptional regulation in the mouse thyroid, kidney cortex and medulla, liver, lungs, and spleen with regard to diurnal variation from morning to afternoon.
In our previous study on nude mice, the absorbed dose to kidney cortex was 47 Gy after administration of 120 MBq 177Lu-octreotate [27], which is only about 10% higher than the mean absorbed dose to the kidney from homogeneous 177Lu distribution.
TEM analysis of the kidney cortex of mice 5 hr after painting with R848 confirmed the recruitment of both monocytic cells and granulocytes in the vasculature.
When we stained frozen kidney cortex sections of control mice and mice 4 weeks after STZ-induced diabetes, we found a strong upregulation of glomerular PKCα expression (Fig. 1A).
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