Sentence examples for mixture was preincubated from inspiring English sources

Twenty-five microfiters of mixture was preincubated with 75 μL preincubation solution (50 mmol/L Tris HCl buffer pH 7.0 containing 1 mmol/L glutathione and 50 mmol/L MnCl2) at 37°C for 30 minutes.

The reaction mixture was preincubated at 35°C for 5 min and the reaction was carried out by addition of 20 μl of the enzyme solution for 10 min (standard assay conditions).

The mixture was preincubated on ice at 4°C overnight.

This reaction mixture was preincubated on ice for 30 45 min.

Before the addition of acetaldehyde, the mixture was preincubated for 10 min at 25°C.

Prior to the addition of Tk-SP, the reaction mixture was preincubated at the respective temperatures for 5 min.

After the mixture being preincubated at 37°C for 5 min, 50  μL 7.6 mmol/L HHL substrate solution, which was solved in 50 mM sodium borate buffer and 6.8 mM NaCl at pH 8.3, was added.

The assay mixtures were preincubated until a constant baseline was obtained and the reaction was started by adding 125 µM insulin.

GCase activities in the tissue and cell lysates were determined as described with 4-methylumbelliferryl β-D-glucopyranoside (4 MU-Glc, 4 mM) as substrate in 0.25% sodium taurocholate and 0.25% Triton X-100 [8]. Assay mixtures were preincubated in the presence and absence of the conduritol B epoxide (CBE, 1 mM), a specific irreversible inhibitor of GCase, for 30 min at 37°C.

Mixtures were preincubated at 37°C for 30 minutes.

Before adding MBP-RacA, the mixtures were preincubated for 15 min at room temperature.