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The reaction mixture was kept in a 45 ℃ water bath for 12 h.
Total cDNA in the transfection mixture was kept constant at 4 μg, except for Western-blotting experiments (6 μg).
The mixture was kept for 5 min.
This mixture was kept in the shaker for 24 h.
The mixture was kept at room temperature for 20 h.
Such mixture was kept at room temperature under continuous stirring.
The mixture was kept stirred in an ice bath.
The reaction mixture was kept in ice bath.
The mixture was kept at 40°C with stirring overnight.
The resulting mixture was kept at 0 °C for 24 h.
The mixture was kept at 4°C for 4 h with constant shaking at 300 rpm.
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