The hydrolyzed mixture was separated by centrifugation at 8,000 rpm for 30 min, and the separated supernatant was heat-deactivated by heating the hydrolysate for 15 min at 90°C in a water bath and filtered with a 0.22-μm sterile filter (Millipore Stericup®, Millipore™, Billerica, MA, USA).
The obtained mixture was separated and purified to examine the decomposed products.
A 30-mg amount of binary isomer mixture was separated on an analytical column.
The first-round RT-PCR reaction mixture was separated from the second-round PCR mixture by silicone oil.
After the first reaction, the solid present in the reaction mixture was separated using filtration, and then the toluene and water layers were separated using a separating funnel.
In this toroidal column the protein mixture was separated with the locations of the protein peaks being consistent with those predicted by the partition coefficients.
Subsequently, after the PCL/NaCl mixture was separated from the mold, the PCL/NaCl mixture was soaked in D.I. water for 24 h to leach out the NaCl particles.
Next, experiments were performed wherein a propane/oxygen mixture was separated from a propane/air mixture by a thin diaphragm to observe the transmission of an overdriven detonation wave.
The soil and oily waste mixture was separated and weighted.
After reaction, the mixture was separated under magnetic field.
