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The mixture was strained through filter paper.
After 24 h, the mixture was strained, the marc was pressed and the liquid was filtered and stored in refrigerator at 4 °C.
The resulting mixture was strained through a 70-µm filter (BD Biosciences, Franklin Lakes, NJ), and filtered cells were re-suspended in culture medium consisting of DMEM/F12 (Life Technologies, Carlsbad, CA) with 10% fetal bovine serum (Sigma-Aldrich, St Louis, MO) and 1× penicillin/streptomycin (Life Technologies, Carlsbad, CA).
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The mixture then was strained; the marc (the damp solid material) was pressed; after standing decantated and the liquid part was clarified by filtration.
After the mixture cools it is strained and then returned to the heat.
But to make Greek yogurt, the mixture is then strained while still warm to remove the whey and concentrate the yogurt mass.
Once the mixture is cool, strain it, then stir in the remaining three tablespoons of rum.
The ligation mixture was transformed into E. coli strain DH5α.
The pentaplex mixture was tested with 120 clinical strains and also against other Gram positive and Gram negative strains to determine the primers' specificity.
The resulting reaction mixture was used to transform E. coli strain DH10B.
Although the optimized tea mixture was effective against all Salmonella strains, the inhibition was substantially greater for specific serotypes, with differences observed even among strains of the same serotype (p < 0.05).
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