Sentence examples for solution was preincubated from inspiring English sources
Once transferred, the solution was preincubated for 20 min. During the actual screen this preincubation step facilitates diffusion throughout the well and also allows for any covalent or slow binding compounds to interact with the enzyme.
To investigate the impact of ions on amylase stability, the enzyme solution was preincubated with different divalent metal ions (10 mM) viz.
The solution was preincubated with anti-CRT antibody (12.5 μg/mL, Abcam, ab22683) or anti-Fas antibody (1 μg/mL, Santa Cruz Biotechnology Inc., sc-21730, Santa Cruz, CA, USA) at 4°C overnight on a rotating shaker.
GIPC1 protein (15 micrograms/mL final concentration in blocking solution) was preincubated with 27.F7/27.B1 antibody (1.5 micrograms/mL final concentration in blocking solution) for 1 hr at RT.
This solution was preincubated at 25°C for 10 min, after which 250 μL of 1% starch solution in 0.02 M sodium phosphate buffer (pH 6.9) was added at timed intervals and then further incubated at 25°C for 10 min.
To estimate metal ions and EDTA on enzyme activity, equal volumes of the purified laccase solution were preincubated with metal ions or EDTA solutions (at concentrations of 2.5, 5.0, 10, and 20 mM, resp).
To count the number of sperm fused per oocyte, 'zona-free' oocytes prepared by immersion in acidic Tyrode's solution were preincubated with DAPI and then subjected to IVF, as shown in Fig. 7A.
Particle suspensions prepared in different buffer solutions were preincubated at 25°C for 5 min before each measurement.
Inhibitor and enzyme solutions were preincubated together for 15 min at room temperature prior to the assay, in order to allow the formation of enzyme-inhibitor complex.
The enzyme and the substrate solutions were preincubated, mixed, and followed by the enzymatic reaction at the same temperature.
Enzyme and inhibitor solutions were preincubated prior to assay for 15 min (at room temperature), in order to allow for the formation of the E-I complex.
