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The product mixture was sampled and analyzed.
Reaction mixture was sampled at appropriate time, and added into equal volume of 0.2 M NaOH.
The mixture was sampled at different times and centrifuged for 5 min to discard any sediment.
After every given irradiation time, the mixture was sampled and centrifuged for 14 min at a rate of 11,000 rpm.
A small flowrate of gas-solid mixture was sampled from just above the bed surface at a velocity high enough to ensure turbulent flow in the sampling tube.
Subsequently, an aliquot of 20 µl of the mixture was sampled at 30, 60, 90, 120 and 180 min, respectively, diluted to a volume of 100 µl with sterilized 0.9% saline, and plated onto 3 LB agar plates (30 µl each plate).
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Aliquots (50 μl) of the reaction mixture were sampled at every 1-h intervals, diluted fourfold with distilled water.
Following initiation of the reaction at pH 9.5, aliquots of the reaction mixture were sampled at designated time points and the Av1 specific activity was determined.
The hydrolysis conditions were 50°C and 200 rpm, and mixtures were sampled at different hydrolysis times.
The mixtures were sampled at different time-points and passed through 100 kDa cutoff filters.
We exploited two methods: rapid quench flow by which reaction mixtures are sampled on the millisecond to second time scale [13] and stopped flow which allows reactions to be followed continuously by optical methods [14].
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