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In all tests the minimum overlap size is 1; for all other parameters we use the default values.
It contains pairs of coordinates of fragments of the human genome that fit two criteria: each pair has a minimum overlap size of 1 kb and presents > 90% identity among copies.
Edena was used with default parameters, i.e. minimum overlap size being 50 and coverage cutoff, 4. Total genome coverage from the long and short insert paired-end reads was ~18.25X and ~82.55X (Additional file 1), respectively.
When the length of the suffix is at least the minimum overlap size, τ, we determine the interval for the reads that have a prefix matching the suffix of X and output an overlap record for each entry (using the subroutine outputOverlaps).
When the criteria for sequence assembly was lowered from 99.99% to 99.85% identity, and the minimum overlap size between BACs decreased from 30 kb to 20 kb, we obtained another large contig (~2.2 Mb) from different Y-BACs displaying similar dot-plot and repeat pattern as the 1.2 Mb contig.
Similar(55)
However, its FPR increases with decreasing overlap size, regardless of the minimal overlap size setting.
As such, the Oligo-set creation feature of the gene-to-oligo algorithm requires five user defined oligonucleotide parameters: i) a maximum oligo size, ii) a minimum oligo size, iii) a target overlap size between any top and bottom strand oligos, iv) the minimum Gibbs free energy (ΔG) allowable for intramolecular oligonucleotide folding, and iv) the salt concentration present in the PCR reactions.
The assembly parameters for MIRA for the PGM data were, minimum overlap = 17 bps, minimum contig size =150 bps, minimum neighbor quality needed for tagging = 20, minimum read length =80.
Other user-provided parameters that limit the supersplat alignment algorithm are the minimum and maximum allowable intron sizes and the minimum overlap of a read on a flanking exon.
In the long march procedure, a step size can be selected that creates the minimum overlap between adjacent steps necessary for correct assembly given the read length and dataset size.
The reads were assembled using short-read transcriptome assembler Trans-Abyss (Birol et al., 2009) version 1.2.0, using k-mer sizes of 26 and 49 the minimum overlap length between two reads to be combined in a contig.
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minimum alignment size
minimal overlap size
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