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This study demonstrated that the optimized excitation wavelength, z-step size, image analysis threshold value, and minimum sampling size parameters for the CLFM method are suitable for different types of oil.
The minimum sampling size was calculated using single proportion formula.
AR corrected for the minimum sampling size was computed using F stat version 2.9.3.2 (Goudet 1995), and observed heterozygosity (Hobs) was computed using GENETIX version 4.05.2 (Belkhir et al. 2002).
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The minimum sample size = 382.
The suggested minimum sample size was 82 (41 patients per group).
The result was a minimum sample size of 96 patients.
Above 6,820 runners, the minimum sample size is 341 runners.
Minimum sample size increases with accuracy and is negatively correlated with tree density.
The minimum sample size required to perform the study was estimated at 105 people.
However, a minimum sample size of 36 was suggested by Makridakis et al. (1998).
We considered five as a minimum sample size from each village.
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