Your English writing platform
Discover LudwigSuggestions(5)
Exact(54)
Then overlapping between sequences were detected by BLAT56 with minimum overlap length set to be 3,000 bp.
Clustering was performed using the following variable parameters: minimum percent identity for overlaps (94%), minimum overlap length (30 nt) and maximum length of unmatched overhangs (30 nt).
We took all of the 454 reads as singletons (i.e. neglecting the paired end information) and assembled them using Roche's assembler (Newbler) with default parameters (minimum overlap length 40, minimum overlap identity 90%).
Parameters used were a minimum overlap length of 30 bp with 94% sequence identity.
The 7846 raw DArT sequences were assembled using Sequencher with a minimum overlap length of 50 nucleotides.
The assembly parameters were a minimum overlap length of 40 bp and a minimum overlap identity of 95%.
Similar(6)
De novo assemblies were run using the transcriptome (-cdna) option, minimum read length (-minlen) of 40 nt, isotig length threshold (-icl) of 40 nt, a large contig threshold (-l) of 100 nt, plus a factorial arrangement of the following parameters: minimum overlap lengths of 35 and 45 nt; alignment difference scores of -2 and -6; and minimum overlap identities of 82%to98%82%to98%
ESTs larger than 100 bp length and Q20 quality after removing vectors, adapters, and ploy-A tails were performed to generate a unigene set using Cap3 [ 56] with the minimum overlapping length parameters >45 bp and overlapping identity percentage >90%.
Incremental de novo assembly analysis was performed with a parameter setting requiring a minimum sequence overlap length of 40 nt, a minimum overlap identity of 90% and using 16 CPU cores.
In order to make the results equivalent to those from other OLC assemblers, the parameters were set to a minimum contig overlap length MIN_LEN = (60/100) and a minimum contig overlap identity percentage MIN_IDENT = (95/98).
As separate de novo assembly (of B1K2 and B1K30) was performed using the following parameters: -cdna (to assemble transcripts), -ml 90% (minimum overlap read length, default 40 bp), -urt (to extend contigs using the ends of single read) and -v (a fasta file database for trimming of the contaminants: adapters, primers and poly-A/T).
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com