Sentence examples for min before performing from inspiring English sources

To ensure a steady state travel time, we kept the P-T conditions of the experiment constant for 30 to 180 min before performing the velocity measurements.

These drugs were added to the bath 30 min before performing the phenylephrine concentration-response curves.

To test whether CXCL12 could overcome the inhibitory effect of DP on T cell blast-endothelial cell adhesion, we preincubated activated bEnd.3 cells with CXCL12 (250 ng/ml) and DP (100 µM) for 45 min before performing the adhesion assay.

Samples were stained for 40 to 60 min before performing flow cytometry.

Y27632 was administered at 1.5 mg/kg, intaperitoneally, 20 min before performing operations.

Laemmli buffer was added and the sample was boiled at 95°C for 5 min before performing western blot analyses.

Isolated CD8+ T-cell subsets were either left untreated or exposed to 10 μg mL−1 etoposide for 60 min as well as given time to repair the induced DNA damage after removal of etoposide for 15, 30, 45, 60, or 75 min, respectively, before performing the automated FADU assay as described elsewhere (Moreno-Villanueva et al., 2009).

After completing the ETI procedure, participants had a 30-min break before performing intubation using another laryngoscope.

After the last feeding to the proboscis, a resting period of 90 min was introduced before performing CS and US tests.

After 3 h, the first sample was injected in the LC MS/MS for analysis.The urine/plasma samples were centrifuged at 13,600 rpm for 15 min at 10 °C before performing the derivatization reaction.

Briefly, cells were seeded in α-MEM supplemented with 10% FBS until reaching 80-90% confluence and then incubated for 2 h with or without trypsin (200 nM) for 30 min at 37°C before performing the test according to the procedure proposed by the manufacturer.