Sentence examples for min before stage from inspiring English sources

The embryos were fixed 30 60 min before stage 10 in MEMFA overnight at room temperature, washed thoroughly, and stored in 100% methanol (−20°C) for at least 3 days.

A mixture of Wnt8 mRNA and Oregon Green dextran was injected into a C4 blastomere of a normal embryo (Nakamura & Kishiyama 1971) at the 32-cell stage, reared until 30 60 min before the onset of gastrulation (30 60 min before stage 10), and the embryo was fixed overnight in MEMFA.

Sleep was scored in 20 s epochs starting from 20 min before the first stage 2 epoch.

The dummy was replaced with working equipment (50 mm Mylar speaker) 30 min before the late-stage experiment started.

30 min before start of behavioral testing.

For the study using second-order conditioning, other groups of animals were each injected with 3 μl of saline or saline containing 1 μM epinastine or 500 μM fluphenazine at 30 min before the first conditioning stage, before the second conditioning stage or before the final test in second-order conditioning.

After successful completion of each stage horses were rested for at least 5 min before moving on to the next stage.

Before stage 12, Astana held ninth overall.

Scanning is performed every 40 min for the early stage and then every 60 min before there is a water breakthrough at the outlet end of core.

At the stage immediately (5 min to 10 min) before the beginning of inversion, a V. globator embryo is fully cleaved.

The autophagosome is a transient organelle existing for less than 10 min before fusing with the lysosome, resulting in the appearance of autophagolysosomes at various stages of degradation [ 131].