Sentence examples for min before removing from inspiring English sources

For exp II, COCs were incubated for 30 min before removing oocytes.

Remove the muffin tin from the oven, and allow it to sit for 5 min. before removing the muffins.

The sample was kept at high-temperature for 35 min before removing the furnace and collecting the second set of CT scans.

After reaction at 30 °C with agitation at 200 rpm for 20 min, 100 μL of 1 M HCl was added to stop the reaction, and centrifugation was performed at 13,000×g for 10 min, before removing 500 μL of sample for reverse-phase high-performance liquid chromatography analysis using a Zorbax® SB-Aq column (250 × 4.6 mm, 5 µm; Agilent Technologies, USA) at a detection wavelength of 210 nm (Ni et al. 2013).

After the microinjection, the injection cannula was left in place for an additional 2 min before removing the cannula.

The mixed suspension was then incubated at room temperature for 60 min with constant rotating and then inserted into a magnet for 2 min before removing the supernatant.

The crude extract was sonicated on ice and then centrifuged at 13,000 rpm for 10 min at 4°C before removing the supernatant and storing at −20°C.

The grids were then left for another 1 min before gently removing excess liquid with a filter paper.

After 120 hr, the assay was terminated by placing the plates on ice for 1 min before gently removing the experimental media and replacing it with 200 μL ice-cold 10% (wt/vol) trichloroacetic acid, 10% (wt/vol).

Each oligonucleotide probe was radioactively labeled in 5μl reactions (2.5 pmole of oligo, 5U T4 PNK, and 33 nmol ATP33 (10μCi)) for 45 min at 37°, terminated by heating to 80° for 15 min, and then pooled before removing unincorporated ATP using MicroSpin G-25 columns (AMERSHAM BIOSCIENCES).

Further centrifugation at 400  g for 7 min was carried out before removing the upper layer of plasma with a sterile pastette (without disturbing the lower levels).