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The samples were ultrasonicated for 15 min before using.
To avoid aggregation, these suspensions were ultrasonically processed for 15 min before using.
The obtained C60 dispersion was stored in a 4°C refrigerator and dispersed by ultrasound for 30 min before using.
The fresh working solution was prepared by mixing 25 mL acetate buffer, 2.5 mL TPTZ solution, and 2.5 mL FeCl3·6H2O solution and then equilibrating at 37°C for 15 min before using.
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Stock suspensions were diluted to achieve required concentrations and sonicated (three 1 min cycles) before use.
The polyplex formulation was incubated at room temperature for 30 min before used.
The test suspensions were sonicated for 20 min before use.
Silica particles were sonicated for 5 min and vortexed for 1 min before use.
The reactor, hydration flask and all tubing were autoclaved at 121 °C for 20 min before use.
Firstly, the collected BoNT-negative sera were kept in room temperature for 10 min before use.
The gold-coated substrate was then annealed in H2 flame for 1 min before use.
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